NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE60729 Query DataSets for GSE60729
Status Public on Nov 01, 2015
Title Gene expression profile of HIF-1α silenced or non-silenced human DCs stimulated with A. fumigatus under normoxic or hypoxic conditions
Organism Homo sapiens
Experiment type Expression profiling by array
Summary DCs play a central role for the immune response against the mold Aspergillus fumigatus. Hypoxic microenvironments occur during infection with A. fumigatus. Hypoxia and signaling via hypoxia inducible factor 1α may modulate the response of DCs; however, the role in fungal infections is unclear.
We used microarrays to determine the influence of hypoxia and HIF-1α signaling on the immune response of human DCs towards A. fumigatus.
 
Overall design HIF-1α silenced or non-silenced, human monocyte-derived DCs were cultivated for 6 h in normoxia or hypoxia (1 % O2) without stimulation or stimulated with inactivated germ tubes of A. fumigatus. Three independent experiments with DCs derived from different blood donors were performed. RNA was extracted and hybridized on Affymetrix microarrays.
 
Contributor(s) Fliesser M, Loeffler J, Bonin M
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 25, 2014
Last update date Mar 21, 2019
Contact name Mirjam Fliesser
Phone +4993120136408
Organization name University Hospital Wuerzburg
Department Medical Clinic II
Lab AG Loeffler
Street address Josef-Schneider-Str. 2
City Wuerzburg
State/province Bavaria
ZIP/Postal code 97080
Country Germany
 
Platforms (1)
GPL13667 [HG-U219] Affymetrix Human Genome U219 Array
Samples (24)
GSM1486351 DCs_normoxia_nonsilenced_unstimulated_rep1
GSM1486352 DCs_normoxia_nonsilenced_unstimulated_rep2
GSM1486353 DCs_normoxia_nonsilenced_unstimulated_rep3
Relations
BioProject PRJNA259372

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60729_RAW.tar 46.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap