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| Status |
Public on Apr 11, 2016 |
| Title |
Gene expression profiles of erythroid progenitors treated with vehicle or the HDAC1/2-selective inhibitor ACY-957 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Determine the effects of HDAC1/2-selective chemical inhibtion on the gene expression profiles of erythroid progenitors derived from human CD34+ bone marrow cells.
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| Overall design |
CD34+ cells isolated from human bone marrow of healthy normal donors were cultured in a 2-phase system. This system has been described previously (Sankaran, Menne et al. 2008). Cells were expanded for 7 days in expansion media, followed by an additional 5 days culture in erythroid differentiation media in the presence of vehicle or ACY-957 (an HDAC1/2-selective inhibitor). Experiments were conducted in triplicate for each condition. Each replicate experiment utilized cells from different donors.
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| Contributor(s) |
Shearstone JR, Golonzhka O, Chonkar A, Tamang D, van Duzer JH, Jones SS, Jarpe MB |
| Citation(s) |
27073918 |
| Submission date |
Aug 26, 2014 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Jeff Shearstone |
| E-mail(s) |
jshearstone@acetylon.com
|
| Phone |
617-245-1321
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| Organization name |
Acetylon Pharmaceuticals
|
| Department |
Biology
|
| Street address |
70 Fargo Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02210 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE60793 |
Chemical inhibition of Histone Deacetylases 1 and 2 induces fetal hemoglobin through activation of Gata2 |
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| Relations |
| BioProject |
PRJNA259551 |
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