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| Status |
Public on Jan 10, 2009 |
| Title |
Expression data from Murine T cell in response to cycloheximide treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cycloheximide has been used for inhibiting protein synthesis in order to identify IL-2 direct targeted genes in T cells, but the effects of cycloheximide itself on genomewide genes are not well defined
We conducted a global analysis of cycloheximide regulated genes in mouse T cell line CTLL-2 using Affymetrix mouse genome array, and identified 213 genes differentially expressed (?1.5-fold change) in response to cycloheximide 30min after adding cylcoheximide to T cells cultured without IL-2 for 14 hours. These genes involve in a number biological processes.
Keywords: stress response
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| Overall design |
Murine cytotoxic T lymphocyte cell line CTLL-2, derived from a C57BL/6 mouse, is an IL-2 dependent cell line. Cells were cultured in D10 medium in an incubator with 5% CO2 at 37oC to mid-log phase (~2 X 105 cells/mL), and collected by centrifugation, washed with phosphate buffered saline (PBS) (Invitrogen), resuspended at 1 X 106 cells/mL in the medium without IL-2 (IL-2 starvation) and cultured for 14 hours (No IL-2 stimulation, T0, 5 reps), followed by adding cycloheximide (20 ?g/mL) for 30min (T4-CHX, 4 reps). Total RNA were extracted from the cells and was hybridized on Affymetrix GeneChip Mouse Genome 430 2.0 Array.
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| Contributor(s) |
Zhang Z, Martino A, Faulon J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 19, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Zhaoduo Zhang |
| E-mail(s) |
zzhang@sandia.gov
|
| Phone |
925-294-2513
|
| Fax |
925-294-3282
|
| Organization name |
Sandia National Labs
|
| Department |
Biosystems
|
| Street address |
7011 East Ave
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94551 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM108343 |
T cell_IL-2_Cycloheximide_4 hour_rep1 |
| GSM108344 |
T cell_IL-2_Cycloheximide_4 hour_rep2 |
| GSM108345 |
T cell_IL-2_Cycloheximide_4 hour_rep3 |
|
| Relations |
| BioProject |
PRJNA97555 |
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