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| Status |
Public on May 08, 2017 |
| Title |
Genomewide poly(A) site mapping for YJR141W and related mutants |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Genomewide characterization of YJR141W role in mRNA polyadenylation.
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| Overall design |
Poly(A) site usage was quantified using the 3'T-fill aproach for 3 mutants and a control (BY4741) S. cerevisiae strain. Biological duplicates were analyzed for tsa685 (pcf11-2::KanR; his3^1 leu2^0 ura3^0 met15^0 pcf11-2 BY4741) and tsa801 (CFT2-1::KanR; his3^1 leu2^0 ura3^0 met15^0 cft2-1 BY4741). Only one replicate was analyzed for tsa1248 (YJR141W-PH::KanR; his3^1leu2^0 ura3^0 met15^0 yjr141w-ph BY4741) and BY4741 (Mata; his3^1leu2^0 ura3^0 met15^0).
A second batch of libraries (marked as "B") the same starting RNA was performed. The second batch contains biological triplicates for BY4741 and tsa801; and biological duplicates for tsa685 and tsa1248. Samples sharing the same biological origin are labeled with the same roman numeral.
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| Contributor(s) |
Pelechano V, Costanzo M, Boone C, Steinmetz LM |
| Citation(s) |
27708008 |
| Submission date |
Aug 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
vicente.pelechano.garcia@ki.se
|
| Organization name |
ScilifeLab - Karolinska Institutet
|
| Department |
MTC
|
| Street address |
Nobels väg 16
|
| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA259880 |
| SRA |
SRP045904 |
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