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Series GSE61012 Query DataSets for GSE61012
Status Public on Nov 10, 2014
Title Principles of ER Co-Translational Translocation Revealed by Proximity-Specific Ribosome Profiling
Organisms Saccharomyces cerevisiae; Homo sapiens
Experiment type Other
Summary Localized protein synthesis is a fundamental mechanism for creating distinct subcellular environments. Here we developed a generalizable proximity-specific ribosome profiling strategy that enables global interrogation of translation in defined subcellular locations. We applied this approach to the endoplasmic reticulum (ER) in yeast and mammals. We observed the vast majority of secretory proteins to be co-translationally translocated, including substrates capable of post-translational insertion in vitro. Distinct translocon complexes engaged nascent chains at different points during synthesis. Whereas most proteins engaged the ER immediately following or even before signal sequence (SS) emergence, a class of Sec66-dependent proteins entered with a looped SS conformation. Finally, we observed rapid ribosome exchange into the cytosol following translation termination. These data provide insights into how distinct translocation mechanisms act in concert to promote efficient co-translational recruitment.
 
Overall design Ribosome profiling of whole cell or streptavidin-purified ribosomes biotinylated by cytosolic, ER or mitochondrially localized biotin ligase in yeast and mammalian cells
 
Contributor(s) Jan CH, Williams CC, Weissman JS
Citation(s) 25378630, 25378625
Submission date Sep 02, 2014
Last update date May 15, 2019
Contact name Jonathan Weissman
Organization name UCSF
Street address 1700 4th Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13821 Illumina HiSeq 2000 (Saccharomyces cerevisiae)
Samples (32)
GSM1495505 BirA_+CHX_2minBiotin_input
GSM1495506 BirA_+CHX_2minBiotin_pulldown
GSM1495507 BirAHeh2Ssh1_+CHX_7minBiotin_input
Relations
BioProject PRJNA260073
SRA SRP046016

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61012_TableS5.txt.gz 2.2 Mb (ftp)(http) TXT
GSE61012_TableS6.txt.gz 539.1 Kb (ftp)(http) TXT
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