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| Status |
Public on Apr 29, 2015 |
| Title |
ChIP-seq profiling of loci bound by HIF1a in wild-type E12.5 hearts |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: to identify genes directly bound by HIF1a in cardiomycoytes
Methods: crosslinked chromatin isolated from E12.5 mouse hearts was precipitated using an anti-HIF1a antibody and processed for sequencing. A list of genes containing a HIF1a peak (Minimum score 3.5) was compared with RNA-seq results to identify genes that are bound by HIF1a and expressed in E12.5 hearts
Results: this analysis identified 1016 genes expressed and bound by HIF1a in embryonic hearts
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| Overall design |
Two experimental replicates and one input
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| |
|
| Contributor(s) |
Guimarães-Camboa N, Carlos Zambon A |
| Citation(s) |
26028220 |
| Submission date |
Sep 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexander C Zambon |
| Organization name |
Keck Graduate Institute
|
| Department |
Biopharmaceutical Sciences
|
| Lab |
Zambon
|
| Street address |
517 Watson Drive
|
| City |
Claremont |
| State/province |
CA |
| ZIP/Postal code |
91711 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA260618 |
| SRA |
SRP046747 |
