|
| Status |
Public on Feb 12, 2015 |
| Title |
Cohesin regulates gene expression through spatial clustering of enhancer elements |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
H3K27Ac ChIP-seq in wild type and cohesin-deficient thymocytes
|
| |
|
| Overall design |
Rad21 was deleted in CD4+ CD8+ double positive (DP) thymocytes by crossing a Rad21 floxed allele with a Cd4-driven Cre transgene. DP positive thymocytes were FACS-sorted from control and Rad21-/- littermates, which were then used to perform chromatin immunoprecipitation for histone H3 acetylated on lysine 27 (H3K27Ac).
|
| |
|
| Contributor(s) |
Merkenschlager M |
| Citation(s) |
25677180 |
| Submission date |
Sep 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gopuraja Dharmalingam |
| Organization name |
MRC London Institute of Medical Sciences
|
| Department |
Epigenetics section
|
| Street address |
Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA261059 |
| SRA |
SRP047133 |
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