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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 16, 2014 |
| Title |
Deletion of human tarbp2 reveals cellular microRNA targets and cell cycle function of TRBP |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
TRBP has two known functions as Dicer co-factor and PKR inhibitor. However, the role of TRBP in miRNA biogenesis is controversial and its regulation of PKR in mitosis remains unexplored. Here, we generate TRBP KO HeLa cells and find that TRBP depletion alters Dicer processing sites of a subset of miRNAs, but does not affect Dicer stability, miRNA abundance, or Argonaute loading. By generating PACT, another Dicer interactor, and TRBP/PACT double-KO cells, we further show that TRBP and PACT do not functionally compensate each other and that only TRBP contributes to Dicer processing. We also report that TRBP is hyperphosphorylated by JNK in M phase when PKR is activated by cellular dsRNAs. Hyperphosphorylation potentiates the inhibitory activity of TRBP on PKR, suppressing PKR in M-G1 transition. By generating the first human TRBP KO, our study clarifies the role of TRBP and unveils negative feedback regulation of PKR through TRBP phosphorylation.
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| Overall design |
small RNAs of wild type, TRBP knockout, PACT knockout and TRBP/PACT double knockout cells were sequenced by Illumina Miseq.
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| Contributor(s) |
Kim Y, Yeo J, Lee J, Seo D, Kim VN |
| Citation(s) |
25437560 |
| Submission date |
Sep 16, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Daekwan Seo |
| E-mail(s) |
daekwan.seo@psomagen.com
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| Phone |
+1-301-251-1007
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| Organization name |
Psomagen Inc
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| Department |
Bioinformatics
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| Lab |
BI
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| Street address |
1330 Piccard Dr., Ste 103
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| City |
Rockville |
| State/province |
MARYLAND |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA261157 |
| SRA |
SRP047169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61458_RAW.tar |
60.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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