|
| Status |
Public on Nov 10, 2014 |
| Title |
Delineating Tumor-infiltrating Antigen Presenting Cell populations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to compare tumor-infiltrating antigen presenting cell populations by global transcriptome profiling (RNA-seq) to help further delineate sub-populations of infiltrating myeloid cells in tumor.
Methods: Four tumor antigen presenting cell populations were sorted from digested B78chOVA (melanoma variant) tumors in biological triplicate
Results: RNA was extracted from the 4 groups (n=3 per group) and prepared for RNAseq. Sequencing yielded ~405 million reads with an average read depth of 33.7 million reads/sample. Reads were then aligned to the mouse genome (UCSC mm10) and those that mapped uniquely to known mRNAs were used to assess differential expression.
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| |
|
| Overall design |
Examination of four tumor infiltrating myeliod populations
|
| |
|
| Contributor(s) |
Krummel MF, Broz M, Binnewies M |
| Citation(s) |
25446897 |
| Submission date |
Sep 16, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Joshua Pollack |
| E-mail(s) |
joshua.pollack@pionyrtx.com
|
| Organization name |
Pionyr Immunotherapeutics
|
| Street address |
2 Tower Pl. Ste 800
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA261162 |
| SRA |
SRP047171 |
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