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| Status |
Public on Sep 12, 2015 |
| Title |
RNA-Sequencing analysis of the Drosophila transcriptomic response to infection by entomopathogenic nematodes and their mutualistic bacteria |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of next generation RNA sequencing to analyze the transcriptional response of Drosophila adult flies to infection by the insect pathogenic nematodes Heterorhabditis bacteriophora and their mutualistic bacteria Photorhabdus luminescens, either separately or together. We find that Heterorhabditis and Photorhabdus differentially modulate a large number of genes, many of which participate in metabolic functions, stress responses, repression of gene transcription and neuronal activities. We have also identified Drosophila genes with potential role in nematode recognition and others with putative anti-nematode properties. These findings generate novel insights into how the host immune function is shaped to respond against nematode parasites and their associated bacteria.
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| Overall design |
Transcriptional profiles of Drosophila wild-type adult flies infected with Heterorhabditis bacteriophora carrying or lacking Photorhabdus or the bacteria alone were generated at 12 and 30 hours post infection using Illumina deep sequencing technology.
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| Contributor(s) |
Eleftherianos I, Castillo JC |
| Citation(s) |
26162375 |
| BioProject |
PRJNA260516 |
| Submission date |
Sep 16, 2014 |
| Last update date |
Jun 10, 2019 |
| Contact name |
Priti Kumari |
| E-mail(s) |
pkumari@som.umaryland.edu
|
| Phone |
410-706-8353
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| Organization name |
University Of Maryland Baltimore School Of Medicine
|
| Department |
Institute For Genome Sciences
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| Street address |
801 W Baltimore St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (7)
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| GSM1505447 |
Dmel_infected_Axenic_Heterorhabditis_Bacteriophora_12h |
| GSM1505448 |
Dmel_infected_Axenic_Heterorhabditis_Bacteriophora_30h |
| GSM1505449 |
Dmel_infected_Symbiotic_Heterorhabditis_Bacteriophora_12h |
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| Relations |
| SRA |
SRP047068 |
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