|
| Status |
Public on Oct 20, 2014 |
| Title |
Cooperativity, specificity and evolutionary stability of Polycomb targeting in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
We report the evolutionary behaviour of Polycomb group proteins, their recruitment factors and their underlying sequences by performing ChIP-seq analysis in 4-5 different Drosophila species (GSE60428) and HiC analysis in Drosophila melanogaster. We demonstrate an extremely high conservation of Polycomb repressive domains across Drosophila species We validate few cases of PRE divergence that shows that cis-driven PRE evolution is a rare event. We further show that PHO recruitment to Polycomb domains is evolutionarily robust to motif changes and that PRC1 stabilizes binding of its key recruiter
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| |
|
| Overall design |
HiC experiments in wild type drosophila embryos
|
| |
|
| Contributor(s) |
Schuettengruber B, Elkayam NO, Sexton T, Entrevan M, Stern S, Thomas A, Yaffe E, Parrinello H, Tanay A, Cavalli G |
| Citation(s) |
25284790 |
| Submission date |
Sep 16, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jia-Ming Chang |
| E-mail(s) |
chang.jiaming@gmail.com
|
| Organization name |
National Chengchi University
|
| Department |
Department of Computer Science
|
| Street address |
NO.64, Sec. 2, ZhiNan Rd., Wenshan District
|
| City |
Taipei |
| ZIP/Postal code |
11605 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA261249 |
| SRA |
SRP047189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61471_50_200_top5.domains.txt.gz |
11.1 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_1000.bins.txt.gz |
699.9 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_10000.bins.txt.gz |
92.2 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_10000.n_contact.txt.gz |
13.1 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_16000.bins.txt.gz |
60.5 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_16000.n_contact.txt.gz |
74.9 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_160000.bins.txt.gz |
6.0 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_160000.n_contact.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_20000.bins.txt.gz |
46.4 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_20000.n_contact.txt.gz |
8.1 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_2500.bins.txt.gz |
331.3 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_2500.n_contact.txt.gz |
23.2 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_32000.bins.txt.gz |
28.8 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_32000.n_contact.txt.gz |
19.7 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_40000.bins.txt.gz |
23.2 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_40000.n_contact.txt.gz |
62.8 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_5000.bins.txt.gz |
173.8 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_5000.n_contact.txt.gz |
25.3 Mb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_8000.bins.txt.gz |
115.4 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_80000.bins.txt.gz |
11.9 Kb |
(ftp)(http) |
TXT |
| GSE61471_nm_none_80000.n_contact.txt.gz |
15.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
