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| Status |
Public on Aug 07, 2015 |
| Title |
Human fetal pancreas transcriptome analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterize the transcriptional programs that underlie pancreas differentiation and identity, we have generated genome-scale expression profiles by RNA-seq from human embryonic stem cell derived liver progenitors and human fetal pancreatic tissue (days 54-57 post conception). These samples were compared to those already published transcriptomes (Xie et al., 2013). Together, these samples were used to perform principles compotent analysis. Once this was performed, we were able to identify transcription factors that were important in the identity of each cell type.
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| Overall design |
To generate genome-scale expression profiles by RNA-seq from human embryonic stem cell derived liver progenitors and human fetal pancreatic tissue (days 54-57 post conception), total RNA was isolated from human embryonic stem cell derived liver progenitors and frozen human fetal pancreas. Libraries were sequenced and mapped to the hg19 version of the human genome. Gene expression was determined using Sailfish. These samples were compared to those already published transcriptomes (Xie et al., 2013). Together, these samples were used to perform principles compotent analysis.
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| Contributor(s) |
Xie R, Patel N, Sander M |
| Citation(s) |
26440894 |
| Submission date |
Oct 01, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Maike Sander |
| E-mail(s) |
masander@ucsd.edu
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| Organization name |
UC San Diego
|
| Street address |
2880 Torrey Pines Scenic Dr.
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (2) |
| GSM1517598 |
human_fetal_panc_54to57dpc_RNAseq replicate 1 |
| GSM1517599 |
human_fetal_panc_54to57dpc_RNAseq replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE61948 |
Transcriptome and cistrome analysis reveals synergistic roles for Sox9 and Pdx1 in lineage allocation of foregut progenitor cells |
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| Relations |
| BioProject |
PRJNA262734 |
| SRA |
SRP048556 |
