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| Status |
Public on Jun 15, 2016 |
| Title |
Gene expression profiles of HT29 cells in which REG4 expression was suppressed. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
GATA6 is a zinc finger transcription factor that is required for the proliferation, development and specific gene regulation in the gastrointestinal tract. We have recently reported that GATA6-mediated induction of the intestinal stem cell marker LGR5 is required for the tumorigenicity of colon cancer cells. However, knockdown of LGR5, unlike GATA6, does not affect the proliferation of these cells under adherent conditions. Here we show that REG4, a member of the regenerating islet-derived (REG) family, is a target of GATA6. We further demonstrate that REG4 is downregulated by overexpression of miR-363, which suppresses GATA6 expression. Moreover, we show that GATA6-mediated activation of REG4 causes an acceleration of the growth of colon cancer cells under adherent conditions. These results suggest that GATA6 simultaneously activates the transcription of genes required for growth (REG4) and clonogenicity (LGR5), and the miR-363-GATA6-REG4/LGR5 pathway is critical for colorectal tumorigenesis.
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| Overall design |
HT29 cells were transfected with a siRNA targeting REG4.
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| Contributor(s) |
Matsumura K, Kawasaki Y |
| Citation(s) |
26387746 |
| Submission date |
Oct 14, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Testu Akiyama |
| Organization name |
Tokyo University
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| Department |
Institute of Molecular and Cellular Biosciences
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| Lab |
Laboratory of Molecular and Genetic Information
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| Street address |
1-1-1, Yayoi, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
110-0032 |
| Country |
Japan |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA263823 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62318_RAW.tar |
14.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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