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Status |
Public on Dec 11, 2014 |
Title |
IRF4/BATF and interleukin-33 orchestrate development and maintenance of adipose tissue resident regulatory T cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To understand the differentiation of effector Tregs in more detail, we have performed transcriptional profiling of central Tregs and effector Tregs, based on Blimp1 expression. We performed RNA-sequencing of Foxp3+ regulatory T cells, comparing Blimp1/GFP+ and Blimp1/GFP- cells
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Overall design |
Three biologically independent samples for each condition were sequenced (condition 1: CD4+ CD25high Blimp1/GFP+; condition 2: CD4+ CD25high Blimp1/GFP-); cells were sorted from pooled spleens and lymphnodes of Blimp1/GFP reporter mice
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Contributor(s) |
Kallies A, Shi W, Liao Y, Smyth GK |
Citation(s) |
25599561 |
Submission date |
Oct 21, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Wei Shi |
E-mail(s) |
Wei.Shi@onjcri.org.au
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Organization name |
Olivia Newton John Cancer Research Institute
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Department |
Bioinformatics and Cancer Genomics
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Street address |
Level 5, ONJ Cancer Centre, 145 Studley Rd
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City |
Heidelberg |
State/province |
VIC |
ZIP/Postal code |
3084 |
Country |
Australia |
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Platforms (1) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA264362 |
SRA |
SRP049087 |