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| Status |
Public on Nov 01, 2015 |
| Title |
The evolution of genic piRNA cluster loci expression patterns |
| Organisms |
Drosophila erecta; Drosophila melanogaster; Drosophila virilis; Drosophila yakuba; Oryctolagus cuniculus; Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
This study examines the conservation of Piwi-interacting RNA (piRNA) clusters that come from protein coding gene transcripts. By sequencing small RNA libraries from gonad tissues of Drosophilids and Glires we discover a diverse set of genic piRNA clusters conserved across animals. This dataset reveals new expression patterns for genic piRNA clusters and examines whether changing piRNA expression patterns correlates with sequence changes in piRNA cluster genomic sequence across a variety of animal species.
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| Overall design |
We dissected gonad tissues consisting of ovaries from wildtype adult Drosophilids (D.melanogaster, D.erecta, D.yakuba, D.virilis) and testes from wildtype pre-pubsecent and adult mouse and rats. Adult testes from rabbits were purchased from Pel-Freez Biologicals. Total or immunopreciptated RNAs were extracted from the pulvirized gonad tissues of Drosophilids and Glires. Small RNAs were purified from these samples, converted into cDNA libraries, and sequenced on an Illumina HiSeq2000.
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| Contributor(s) |
Lau NC, Chirn G |
| Citation(s) |
26588211 |
| Submission date |
Oct 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
NELSON LAU |
| E-mail(s) |
nclau@bu.edu
|
| Organization name |
Boston University School of Medicine
|
| Department |
Biochemistry
|
| Street address |
72 E Concord St K219, Dept of Biochemistry
|
| City |
BOSTON |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (7)
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| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL13307 |
Illumina HiSeq 2000 (Drosophila yakuba) |
| GPL13313 |
Illumina HiSeq 2000 (Drosophila virilis) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
| GPL18453 |
Illumina HiSeq 2000 (Oryctolagus cuniculus) |
| GPL19321 |
Illumina HiSeq 2000 (Drosophila erecta) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA264407 |
| SRA |
SRP049103 |
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