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| Status |
Public on Dec 30, 2014 |
| Title |
RNA-Seq Analysis in hES/ iPS cell-derived neuronal samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We characterized the gene expression by Hierarchical Clustering and one-matrix clustering in hESC, day 12 progenitors, day 25-day 27, day82 differentiated hypothalamic neurons from hESCs and day 45 neurons derived from iPSCs generated from controls (2 independent) and BBS (Bardet-Biedl Syndrome, 3 independent) subjects.
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| Overall design |
RNA was isolated from cells of 13 samples (1 hESC, triplicate for day 12 progenitors, 1 day 25 neuron sample, duplicate for day 27 neuron samples, 1 day 82 neuron sample, five day 45 neuron samples made from 5 independent iPSC lines ) using RNeasy Micro Kit (QIAGEN). Quality control of the RNA was carried out with the Agilent Bio-analyzer, Qubit 2.0 at the MPSR of Columbia University. 100 ng of RNA with RIN ≥ 9 were used for generating mRNA-focused libraries using TruSeq RNA Sample Preparation Kit v2 and sequencing on an Illumina 2000/2500 V3 Instrument offered by the Columbia Genome Center.
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| Contributor(s) |
Wang L, Egli D, Leibel RL |
| Citation(s) |
25555215 |
| Submission date |
Nov 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Liheng Wang |
| E-mail(s) |
lw2381@cumc.columbia.edu
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| Organization name |
Columbia University Medical Center
|
| Department |
Department of Medicine
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| Lab |
Domenico Accili
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| Street address |
1150 Saint Nicholas Avenue, Room 234
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA266234 |
| SRA |
SRP049475 |
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