|
| Status |
Public on Dec 15, 2014 |
| Title |
Enhancer Analysis in Peripheral Nerve Injury |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq of H3K27acetylation in sham and injured nerve. Schwann cells play an important role in the response of peripheral nerve to injury. This study was designed to identify enhancers that are altered in sciatic nerve at 3 days post-injury to help identify pathways that mediate the gene expression reprogramming that occurs in Schwann cells after nerve injury. We employed ChIP-seq analysis of H3K27 acetylation as a mark of actively engaged enhancers, and compared enhancers in the distal stump of transected sciatic nerve compared to contralateral (sham) condition.
|
| |
|
| Overall design |
2 independent ChIP samples each for Sham and Injury conditions, with respective inputs
|
| |
|
| Contributor(s) |
Hung H, Sun G, Keles S, Svaren J |
| Citation(s) |
25614629 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS075269 |
Epigenetic Regulation of Peripheral Nerve Myelination |
UNIVERSITY OF WISCONSIN MADISON |
Svaren |
|
| Submission date |
Nov 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
John Svaren |
| E-mail(s) |
jpsvaren@wisc.edu
|
| Organization name |
University of Wisconsin
|
| Department |
Comparative Biosciences
|
| Lab |
Waisman Center
|
| Street address |
1500 Highland Ave.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA266627 |
| SRA |
SRP049618 |
Less...
More...