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| Status |
Public on Apr 11, 2020 |
| Title |
Procaspase 3-Cyclin D1 assembly rewires cancer cells from apoptosis to proliferation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To gain insights into the activity of Cyclin D1 in tumor maintenance, we developed a cancer cell-specific RNAi strategy to knock down its expression and analyze the outcome on gene expression profile of the entire tumor.
Method: RAS/DNP53-driven tumor mRNA profiles were generated by RNA-Sequencing. Reads from RNA-Seq samples were mapped to the Mus musculus reference genome (MGSCv37) using Bowtie and TopHat. Transcript abundance and differential expression were called by Cufflinks.
Results: Cyclin D1 knock down in RAS-G12V or MMTV-ErbB2-driven tumors leads to expression change of genes involved in developmental processes, but no significant change in apoptotic genes was recorded among the samples.
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| Overall design |
RAS/DNP53-driven tumor mRNA profiles were generated by RNA-Sequencing. Reads from RNA-Seq samples were mapped to the Mus musculus reference genome (MGSCv37) using Bowtie and TopHat. Transcript abundance and differential expression were called by Cufflinks.
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| Contributor(s) |
Bienvenu F, Maurel B, Champagne J, Dubois E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 24, 2014 |
| Last update date |
Apr 12, 2020 |
| Contact name |
Frederic Emile Bienvenu |
| E-mail(s) |
frederic.bienvenu@igf.cnrs.fr
|
| Organization name |
CNRS-Institute of Functinal Genomics
|
| Department |
Cancer Biology
|
| Lab |
Bienvenu
|
| Street address |
141 rue de la Cardonille
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| City |
Montpellier |
| ZIP/Postal code |
34094/05 |
| Country |
France |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
|
| GSM1553112 |
WT RAS/DNP53 HAsiRNA rep1 [140521_SND393_B_L002_HBY-15_R1] |
| GSM1553113 |
WT RAS/DNP53 HAsiRNA rep1 [140521_SND393_B_L003_HBY-15_R1] |
| GSM1553114 |
WT RAS/DNP53 HAsiRNA rep2 [140219_SND393_A_L002_HBY-5_R1] |
| GSM1553115 |
WT RAS/DNP53 HAsiRNA rep2 [140219_SND393_A_L003_HBY-5_R1] |
| GSM1553116 |
TAG RAS/DNP53 HAsiRNA rep1 [140521_SND393_B_L002_HBY-17_R1] |
| GSM1553117 |
TAG RAS/DNP53 HAsiRNA rep1 [140521_SND393_B_L003_HBY-17_R1] |
| GSM1553118 |
TAG RAS/DNP53 HAsiRNA rep2 [140521_SND393_B_L002_HBY-18_R1] |
| GSM1553119 |
TAG RAS/DNP53 HAsiRNA rep2 [140521_SND393_B_L003_HBY-18_R1] |
| GSM1553120 |
TAG RAS/DNP53 HAsiRNA rep3 [140219_SND393_A_L002_HBY-9_R1] |
| GSM1553121 |
TAG RAS/DNP53 HAsiRNA rep3 [140219_SND393_A_L003_HBY-9_R1] |
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| Relations |
| BioProject |
PRJNA268299 |
| SRA |
SRP050180 |
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