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| Status |
Public on Aug 03, 2015 |
| Title |
Comparison of poly(A) and capture RNA-seq: controlled degradation in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We compare the performance of two library preparation protocols (poly(A) and exome capture) in in vitro degraded RNA samples
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| |
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| Overall design |
VcaP cell were grown, and treated with MDV3100 (enzalutamide) or DHT (dihydrotestosterone), intact RNA was isolated and samples were prepared in technical triplicates using two library preparation protocol. Also cells were subject to in vitro degradation through incubation of the whole cell lysate in 37C for increasing amounts of time. Following incbation paired capture and poly(A) libraries were prepared.
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| |
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| Contributor(s) |
Cieslik MP, Chinnaiyan AM |
| Citation(s) |
26253700 |
| Submission date |
Dec 12, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Marcin Piotr Cieslik |
| E-mail(s) |
marcin.cieslik@gmail.com
|
| Organization name |
University of Michigan
|
| Department |
Pathology
|
| Lab |
MCTP
|
| Street address |
500 S State St
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA270181 |
| SRA |
SRP051102 |
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