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| Status |
Public on Dec 23, 2014 |
| Title |
Extremely low frequency electromagnetic fields enhance neuronal differentiation of human mesenchymal stem cells on graphene-based substrate |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Graphene-based substrate has efficient neuronal differentiation of hMSCs. Stimulatory effects of graphene on hMSCs neurogenesis can be enhanced by ELF-EMF exposure and it is mediated by enhancement of cell adhesion accompanied by intracellular signal pathway.
We used microarrays to assess up-regulated genes in human gene expression profiles during neurogenesis induced by ELF-EMF exposure on graphene.
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| Overall design |
3 groups (HMSCs grown in neuronal medium on glass, graphene, graphene under ELF-EMF exposure) were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain total human gene expression. To that end, we hand-selected up-regulated gene involved in neurogenesis, extracellular matrix, and cell migration.
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| Contributor(s) |
Lee Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 22, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
YooJung Lee |
| E-mail(s) |
dbwjddldl@gmail.com
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| Organization name |
Dongguk University
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| Department |
Life science
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| Lab |
Molecular immunology
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| Street address |
Department of Life Science, Dongguk University-Seoul, 3–26 Phil-dong, Chung-gu
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| City |
Seoul |
| ZIP/Postal code |
110-715 |
| Country |
South Korea |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA270981 |
