|
| Status |
Public on Jun 25, 2015 |
| Title |
Induction of circular RNA in fetal heart development recapitulated in in vitro differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We discovered induction of circular RNA in human fetal tissues, including the heart. In this study, we were able to recapitulate this induction by in vitro directed differentiation of hESCs to cardiomyocytes, paving the way for future studies into circular RNA regulation.
|
| |
|
| Overall design |
We harvested hESCs at sequential stages of differentiation: undifferentiated (day 0), mesoderm (day 2), cardiac progenitor (day 5) and definitive cardiomyocyte (day 14). We performed RNA sequencing in biological triplicate, with 3-8 technical replicates each.
|
| |
|
| Contributor(s) |
Szabo L |
| Citation(s) |
26076956 |
| Submission date |
Dec 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Linda Szabo |
| Organization name |
Stanford University
|
| Department |
Biochemistry
|
| Lab |
Salzman Lab
|
| Street address |
Beckman Center, B400 279 W. Campus Dr. MC: 5307
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (71)
|
|
| Relations |
| BioProject |
PRJNA270993 |
| SRA |
SRP051472 |
Less...
More...