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| Status |
Public on Sep 24, 2015 |
| Title |
Multi-level omics analysis of gene expression in a murine model of dystrophin loss and therapeutic restoration [mRNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Duchenne muscular dystrophy (DMD) is a classical monogenic disorder, a model disease for genomic studies and a priority candidate for regenerative medicine and gene therapy. Although the genetic cause of DMD is well known, the molecular pathogenesis of disease and the response to therapy are incompletely understood. Here,we describe analyses of protein, mRNA and microRNA expression in the tibialis anterior of the mdx mouse model of DMD. Notably, 3272 proteins were quantifiable and 525 identified as differentially expressed in mdx muscle (P < 0.01). Therapeutic restoration of dystrophin by exon skipping induced widespread shifts in protein and mRNA expression towards wild-type expression levels, whereas the miRNome was largely unaffected. Comparison analyses between datasets showed that protein and mRNA ratios were only weakly correlated (r = 0.405), and identified a multitude of differentially affected cellular pathways, upstream regulators and predicted miRNA–target interactions. This study provides fundamental new insights into gene expression and regulation in dystrophic muscle.
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| Overall design |
3 Wt, 4 mdx and 4 Pip6e-PMO treated mdx mice
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| Contributor(s) |
Roberts TC, Johansson HJ, McClorey G, Godfrey C, Blomberg KE, Coursindel T, Gait MJ, Smith CI, Lehtiö J, EL-Andaloussi S, Wood MJ |
| Citation(s) |
26385637, 26981371 |
| Submission date |
Dec 22, 2014 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Thomas C Roberts |
| E-mail(s) |
thomas.roberts@dpag.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Department of Physiology, Anatomy and Genetics
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QX |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (11)
|
| GSM1571126 |
Tibialis anterior muscle from Wt mouse 1_mRNA |
| GSM1571127 |
Tibialis anterior muscle from Wt mouse 2_mRNA |
| GSM1571128 |
Tibialis anterior muscle from Wt mouse 3_mRNA |
| GSM1571129 |
Tibialis anterior muscle from mdx mouse 1_mRNA |
| GSM1571130 |
Tibialis anterior muscle from mdx mouse 2_mRNA |
| GSM1571131 |
Tibialis anterior muscle from mdx mouse 3_mRNA |
| GSM1571132 |
Tibialis anterior muscle from mdx mouse 4_mRNA |
| GSM1571133 |
Tibialis anterior muscle from Pip6e-PMO treated mdx mouse 1_mRNA |
| GSM1571134 |
Tibialis anterior muscle from Pip6e-PMO treated mdx mouse 2_mRNA |
| GSM1571135 |
Tibialis anterior muscle from Pip6e-PMO treated mdx mouse 3_mRNA |
| GSM1571136 |
Tibialis anterior muscle from Pip6e-PMO treated mdx mouse 4_mRNA |
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| This SubSeries is part of SuperSeries: |
| GSE64420 |
Multi-level omics analysis of dystrophin loss and therapeutic restoration in a murine model |
|
| Relations |
| BioProject |
PRJNA270992 |
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