|
| Status |
Public on Aug 26, 2015 |
| Title |
RNA-seq of human fibroblasts during normal aging and during aging with rotenone perturbation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human fibroblasts at different population doublings were treated with low amounts of rotenone (mild stress) and compared to untreated fibroblasts. Two different cell lines were used (MRC-5, HFF). Illumina sequencing (HiSeq2000) was applied to generate 50bp single-end reads.
Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
|
| |
|
| Overall design |
60 samples: 3 biological replicates for each group: MRC-5 cells at 4 different population doublings (PD) with and without rotenone; HFF cells at 6 different population doublings with and without rotenone
|
| |
|
| Contributor(s) |
Marthandan S, Klement K, Priebe S, Groth M, Platzer M, Hemmerich P, Diekmann S |
| Citation(s) |
26380578 |
| Submission date |
Dec 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
JenAge Project |
| E-mail(s) |
geo-data@jenage.de
|
| Organization name |
Leibniz Institute for Age Research - Fritz Lipmann Institute
|
| Street address |
Beutenbergstr. 11
|
| City |
Jena |
| ZIP/Postal code |
D-07745 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (60)
|
|
| Relations |
| BioProject |
PRJNA271291 |
| SRA |
SRP051599 |
Less...
More...