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| Status |
Public on Aug 19, 2015 |
| Title |
MacroH2A1 ChIP-seq from H-RasV12 expressing senescent IMR90 human primary lung fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Oncogene induced senescence (OIS) is a tumor suppressive mechanism typified by stable proliferative arrest, a persistent DNA damage response and the senescent-associated secretory phenotype (SASP). MacroH2A1, a tumor suppressive histone variant, is upregulated during OIS. Using ChIP-seq, we found that macroH2A1 undergoes dramatic relocalization during OIS. SASP genes are enriched in macroH2A1-containing chromatin and macroH2A1 is a critical component of the positive feedback loop that maintains SASP expression. Endoplasmic reticulum (ER) stress, a feature of OIS that requires macroH2A1, leads to ATM activation. ER stress triggers a negative feedback loop reducing SASP expression by causing the ATM-dependent removal of macroH2A1 from SASP genes. MacroH2A1 represents a critical control point in the regulation of SASP expression during OIS by We demonstrate that SASP gene expression is regulated by the combined actions of a positive feedback loop that requires macroH2A1 and a negative feedback loop where ER stress leads to ATM activation critical for the removal of macroH2A1 from SASP genes and consequently their repression.
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| Overall design |
macroH2A1 ChIP and corresponding input samples from H-RasV12 OIS IMR90 cells were sequenced
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| Contributor(s) |
Gamble MJ, Chen H |
| Citation missing |
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| Submission date |
Dec 31, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew Jon Gamble |
| E-mail(s) |
matthew.gamble@einstein.yu.edu
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| Organization name |
Albert Einstein College of Medicine
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| Department |
Molecular Pharmacology
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| Lab |
Gamble Lab
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| Street address |
1300 Morris Park Ave / Golding 203
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA271454 |
| SRA |
SRP051655 |
