|
| Status |
Public on Oct 20, 2015 |
| Title |
Interferon-γ inhibition of Ebola virus infection [Monocyte-derived macrophage] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Episodic Ebola virus (EBOV) outbreaks, such as the current one in West Africa, emphasize the critical need for novel antivirals against this highly pathogenic virus. Here, we demonstrate that interferon gamma (IFNγ) prevents morbidity and mortality associated with EBOV infection when administered to mice either 24 hours prior to or 2 hours following EBOV infection. Microarray studies with IFNγ-stimulated human macrophages identified novel interferon-stimulated genes (ISGs) that inhibit EBOV infection upon ectopic expression. IFNγ treatment reduced viral RNA levels in macrophages to a similar degree as cells treated with the protein synthesis inhibitor, cycloheximide, suggesting that IFNγ treatment inhibits genome replication. As IFNγ treatment robustly protects mice against EBOV infection, we propose that this FDA-approved drug may serve as a useful prophylactic or therapeutic strategy during EBOV outbreaks, contributing to the currently limited arsenal of filovirus antivirals.
|
| |
|
| Overall design |
Total RNA was isolated from monocyte-derived macrophages (MDMs) from healthy individuals differentiated in the presence of 50 ng/mL of M-CSF for 6 days and then treated with or without 20 ng/mL of IFNg for 24 hours.
|
| |
|
| Contributor(s) |
Rhein BA, Powers LS, Anantpadma M, Sakurai Y, Bair T, Miller-Hunt C, Davey RA, Monick MM, Maury W |
| Citation(s) |
26562011 |
| Submission date |
Jan 15, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Thomas B Bair |
| E-mail(s) |
iihg-bioinformatics@uiowa.edu
|
| Organization name |
University of Iowa
|
| Street address |
335 EMRB
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
| GSM1585569 |
Monocyte-derived macrophage control |
| GSM1585570 |
Monocyte-derived macrophage control replicate 1 |
| GSM1585571 |
Monocyte-derived macrophage control replicate 2 |
| GSM1585572 |
Monocyte-derived macrophage control replicate 3 |
| GSM1585573 |
Monocyte-derived macrophage interferon-g inhibited |
| GSM1585574 |
Monocyte-derived macrophage interferon-g inhibited replicate 1 |
| GSM1585575 |
Monocyte-derived macrophage interferon-g inhibited replicate 2 |
| GSM1585576 |
Monocyte-derived macrophage interferon-g inhibited replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE64997 |
Interferon-γ inhibition of Ebola virus infection |
|
| Relations |
| BioProject |
PRJNA272681 |
Less...
More...