|
| Status |
Public on Sep 25, 2015 |
| Title |
Effect in HCT116 cells of 3hr cortistatin A treatment on gene expression. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We characterized the marine natural product cortistatin A (CA) as an inhibitor of CDK8 to determine whether pharmacologic inhibition of CDK8 regulates super-enhancer function and inhibits AML proliferation.
In this series, we examine the transcriptional effect on insensitive HCT116 cells of 3hrs exposure to CA.
|
| |
|
| Overall design |
HCT116 cells were treated in triplicate with either DMSO or CA for 3hrs after which RNA was harvested and prepared for RNA sequencing to assess transcriptional changes.
|
| |
|
| Contributor(s) |
Pelish HE, Liau BB, Odell AT, Dowell RD, Poss ZC, Taatjes DJ, Lemieux ME, Shair MD |
| Citation(s) |
26416749 |
| Submission date |
Jan 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew D. Shair |
| Organization name |
Harvard University
|
| Department |
Chemistry and Chemical Biology
|
| Lab |
Shair
|
| Street address |
12 Oxford Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE65161 |
Mediator kinase inhibition further activates super-enhancer-associated genes in AML |
|
| Relations |
| BioProject |
PRJNA273294 |
| SRA |
SRP052713 |
Less...
More...