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| Status |
Public on Jan 19, 2016 |
| Title |
Diarrhea in lymphocytic colitis: ERK1/2-dependent ENaC dysregulation and claudin-4-, -5- and -8-related barrier defects |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Lymphocytic colitis (LC) causes watery diarrhea. This study aimed to identify the inherent pathomechanisms of epithelial transport and barrier dysfunction and regulatory inputs.
Methods: 8 (4 LC samples and 4 unrelated control samples) biopsies of fresh sigmoid colon were analysed by paired end sequencing (Illumina High Seq 2500)
Conclusions: ENaC-mediated Na+ malabsorption via ERK1/2 and epithelial barrier dysfunction from tight junction downregulation through claudin-4, -5, and -8 are mechanisms causing malabsorptive and leak flux diarrhea in LC resulting from the key effector cytokines TNFa and INFg.
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| Overall design |
Sigmoid colon mRNA profiles from 8 unrelated individuals (4 with LC, 4 as control) were generated using paired end sequencing with Illumina High Seq 2500
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| Contributor(s) |
Barmeyer C, Erko I, Fromm A, Bojarski C, Ploeger S, Richter JF, Loddenkemper C, Dames P, Kerick M, Siegmund B, Fromm M, Schweiger MR, Schulzke J |
| Citation(s) |
26658215 |
| Submission date |
Jan 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Michal Ruth Schweiger |
| E-mail(s) |
mschweig@molgen.mpg.de
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| Organization name |
Max Planck Institute For Molecular Genetics
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| Department |
Lehrach
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| Lab |
Schweiger
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| Street address |
Ihnestrasse 63-73
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| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA273003 |
| SRA |
SRP052615 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65107_LymphycyticColitis_Ratios_RawCounts_CPM.txt.gz |
18.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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