 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 18, 2016 |
| Title |
Genome wide extracellular signal-regulated kinase (ERK) pathway components interaction with human genome |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Several recent studies, including ours, show that most components of signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), that once were thought to act predominantly in cytoplasm, are in fact recruited to chromatin and are integral components of transcriptional complexes. However, their distribution along whole genome remains uncovered. Here we investigate genome wide recruitment of the ERK pathway components using chromatin immunoprecipitations (ChIP) followed by deep sequencing, ChIP-Seq.
|
| |
|
| Overall design |
Hela-S3 cells were starved for 48 hours (control) and stimulated with EGF at concentration of 100ng/mL for 20 minutes. Cells were fixed with formaldehyde, chromatin isolated and subjected to ChIP reaction. Two technical replicates of ChIP reaction followed by sequencing were performed.
|
| |
|
| Contributor(s) |
Mikula M, Goryca K, Ledwon J, Skrzypczak M, Grzelak M, Dabrowska M, Kulecka M, Ginalski K, Bomsztyk K, Ostrowski J |
| Citation(s) |
27587583 |
| Submission date |
Jan 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Krzysztof Goryca |
| Organization name |
Uniwersytet Warszawski
|
| Department |
CeNT
|
| Street address |
Banacha 2c
|
| City |
Warszawa |
| ZIP/Postal code |
02-097 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA273670 |
| SRA |
SRP052844 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65323_macs.Pol2-Egf.vs.Pol2.peaks.bed.gz |
24.5 Kb |
(ftp)(http) |
BED |
| GSE65323_macs.pBRAF-EGF.vs.pBRAF.merged.peaks.bed.gz |
8.3 Kb |
(ftp)(http) |
BED |
| GSE65323_macs.pEGFR-Egf.vs.pEGFR.merged.peaks.bed.gz |
12.4 Kb |
(ftp)(http) |
BED |
| GSE65323_macs.pERK_Egf.vs.pERK.merged.peaks.bed.gz |
33.4 Kb |
(ftp)(http) |
BED |
| GSE65323_macs.pMEK_Egf.vs.pMEK.merged.peaks.bed.gz |
6.9 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...