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| Status |
Public on Feb 18, 2015 |
| Title |
Analyzing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Decoding heterogeneity of pluripotent stem cell (PSC)-derived neural progeny is fundamental for revealing the origin of diverse progenitors, for defining their lineages, and for identifying fate determinants driving transition through distinct potencies. Here we prospectively isolated consecutively appearing PSC-derived primary progenitors based on their Notch activation state. We first isolate early neuroepithelial cells and show their broad Notch-dependent developmental and proliferative potential. Neuroepithelial cells further yield successive Notch-dependent functional primary progenitors, from early and mid neurogenic radial glia and their derived basal progenitors, to gliogenic radial glia and adult-like neural progenitors, together recapitulating hallmarks of neural stem cell (NSC) ontogeny. Gene expression profiling reveals dynamic stage specific transcriptional patterns that may link development of distinct progenitor identities through Notch activation. Our observations provide a platform for characterization and manipulation of distinct progenitor cell types amenable for developing streamlined neural lineage specification paradigms for modeling development in health and disease.
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| Overall design |
Human embryonic stem cells (hESCs) H9 were differentiated into 5 distinct populations of neural precursor cells (NPCs) over a time course of 200 days. Each neural precursor populations was then sorted for HES5 expression based on a GFP-HES5 reporter. Both the HES5 positive and HES5 negative populations were then subjected to microarray profiling in singlicate, as well as the hESCs using GeneChipPrimeView Human Gene Expression Array
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| Contributor(s) |
Elkabetz Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 28, 2015 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Michael Johannes Ziller |
| Organization name |
Max Planck Institute of Psychiatry
|
| Department |
Translational Psychiatry
|
| Lab |
Ziller lab
|
| Street address |
Kraepelinstrasse 2-10
|
| City |
Munich |
| ZIP/Postal code |
80804 |
| Country |
Germany |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (11)
|
| GSM1593905 |
human embryonic stem cell line H9 |
| GSM1593906 |
Neuroepithelial cells day 12 positive for HES5 |
| GSM1593907 |
Neuroepithelial cells day 12 negative for HES5 |
| GSM1593908 |
Early radial glial cells day 14 positive for HES5 |
| GSM1593909 |
Early radial glial cells day 14 negative for HES5 |
| GSM1593910 |
Mid radial glial cells day 35 positive for HES5 |
| GSM1593911 |
Mid radial glial cells day 35 negative for HES5 |
| GSM1593912 |
Late radial glial cells day 80 positive for HES5 |
| GSM1593913 |
Late radial glial cells day 80 negative for HES5 |
| GSM1593914 |
Long term neural precursor cells day 220 positive for HES5 |
| GSM1593915 |
Long term neural precursor cells day 220 negative for HES5 |
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| Relations |
| BioProject |
PRJNA273811 |
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