|
| Status |
Public on Feb 01, 2016 |
| Title |
Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Rapid advances in biochemical technologies have enabled several strategies for typing candidate HLA alleles, but linking them into a single MHC haplotype structure remains challenging. Here we have developed a multi-loci haplotype phasing technique and demonstrate its utility towards phasing of MHC and KIR loci in human samples. We accurately (~99%) reconstruct the complete haplotypes for over 90% of sequence variants spanning the 4-megabase region of these two loci. By haplotyping a majority of coding and non-coding alleles at the MHC and KIR loci in a single assay, this method has the potential to assist transplantation matching and facilitate investigation of the genetic basis of human immunity and disease.
|
| |
|
| Overall design |
Complete haplotype phasing of 2 loci (MHC and KIR) in 1 human cell line.
|
| |
|
| Contributor(s) |
Selvaraj S, Schmitt A, Ren B |
| Citation(s) |
26541200 |
| Submission date |
Feb 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony D Schmitt |
| E-mail(s) |
aschmitt1987@gmail.com
|
| Phone |
6178429022
|
| Organization name |
UC San Diego
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Dr. Bing Ren
|
| Street address |
9500 GILMAN DR., BLDG. CMME RM 2071
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA274802 |
| SRA |
SRP053311 |
