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| Status |
Public on Jan 01, 2016 |
| Title |
Gene expression profile in CD4+ T-cell infection with a SIV mutant related to altered humoral immune responses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A mutant simian immunodeficiency (SIVmac239) virus, found to be selected within chronically SIV-infected Burmese rhesus monkeys with relatively enhanced SIV-specific antibody responses, was reconstituted as a molecular clone. The virus (SIV Nef G63E) was then subjected to a preliminary analysis for their intracellular signal transduction and gene expression modulation patterns (as compared with wild type SIVmac239) within infected CD4+ T cells. Analysis implicated that the mutant virus had a moderately enhanced cytopathic phenotype.
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| Overall design |
A SIV mutation (SIVmac239 Nef G63E) found to be enriched in rhesus monkeys with enhanced SIV-specific antibody responses was reconstituted on a pBR-based SIV molecular clone pBRmac239. HSC-F cynomolgus macaque central memory Th2-like T cells were infected with mutant and wild type SIVmac239 at MOI 5 in triplicate along with uninfected controls (i.e. a total of 9 samples) for 24 hours and subjected to analysis of their gene expression patterns.
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| Contributor(s) |
Yamamoto H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 10, 2015 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Hiroyuki Yamamoto |
| Organization name |
National Institute of Infectious Diseases
|
| Department |
AIDS Research Center
|
| Street address |
1-23-1 Toyama
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8640 |
| Country |
Japan |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM1606626 |
HSC-F CD4+ T cell SIVmac239 24h_rep1 |
| GSM1606627 |
HSC-F CD4+ T cell SIVmac239 24h_rep2 |
| GSM1606628 |
HSC-F CD4+ T cell SIVmac239 24h_rep3 |
| GSM1606629 |
HSC-F CD4+ T cell Nef G63E SIVmac239 24h_rep1 |
| GSM1606630 |
HSC-F CD4+ T cell Nef G63E SIVmac239 24h_rep2 |
| GSM1606631 |
HSC-F CD4+ T cell Nef G63E SIVmac239 24h_rep3 |
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| Relations |
| BioProject |
PRJNA275117 |
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