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| Status |
Public on Aug 03, 2015 |
| Title |
Widespread association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growth [gene expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Third-party reanalysis
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| Summary |
The goal of this study was to identify YAP/TAZ direct transcriptional targets and transcriptional partners, through ChIP-sequencing and gene expression profiling.
Keywords: Expression profiling by array
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| Overall design |
MDA-MB-231 cells growing at low confluence in standard growth conditions were transfected with the indicated siRNAs (Dupont et al., Nature 2011). The following day, growth medium was renewed and cells were incubated for 24 more hours, and then harvested for total RNA extraction. 4 independent biological replicates were plated, transfected and harvested in parallel for each siRNA. Raw .CEL files are the same as in GSE59230 (Enzo et al., EMBO J 2015), but gene expression levels have been quantified using Brainarray HGU133Plus2_Hs_ENTREZG_v17 custom chip definition files.
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| Contributor(s) |
Forcato M, Bicciato S, Zanconato F, Cordenonsi M, Piccolo S |
| Citation(s) |
26258633 |
| Submission date |
Feb 19, 2015 |
| Last update date |
Aug 11, 2015 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL17811 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray HGU133Plus2_Hs_ENTREZG_v17] |
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| Samples (12)
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| GSM1614041 |
MDA-MB-231 cells siControl, biological replica A |
| GSM1614042 |
MDA-MB-231 cells siControl, biological replica B |
| GSM1614043 |
MDA-MB-231 cells siControl, biological replica C |
| GSM1614044 |
MDA-MB-231 cells siControl, biological replica D |
| GSM1614045 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica A |
| GSM1614046 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica B |
| GSM1614047 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica C |
| GSM1614048 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica D |
| GSM1614049 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica A |
| GSM1614050 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica B |
| GSM1614051 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica C |
| GSM1614052 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica D |
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| This SubSeries is part of SuperSeries: |
| GSE66083 |
Widespread association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growth |
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| Relations |
| BioProject |
PRJNA275843 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66082_RAW.tar |
56.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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