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| Status |
Public on May 29, 2015 |
| Title |
Human ileum stem cell differentiation by air-liquid interface |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Despite major advances with embryonic and induced pluripotent stem cells or lineage-committed, p63-expressing stem cells of stratified epithelia, we know less about the indigenous stem cells of the gastrointestinal tract, pancreas, liver, and other columnar epithelia which collectively resist cloning in their elemental states. Here we demonstrate the cloning of highly immature epithelial stem cells from defined regions of the human intestine and colon. In this study, we have isolated ileal stem cells and performed air-liquid interface method to induce differentiation of human ileal stem cells. The differentiated structure showed villi-like epithelia which contains enterocytes, goblet cells, endocrine cells and paneth cells.
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| Overall design |
Human ileal stem cells are differentiated by air-liquid interface method and total RNA was collected for microarray preparation.
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| Contributor(s) |
Wang X, Yamamoto Y, Wilson L, Zhang T, Wu L, Ning G, Hong Y, Khor CC, Howitt B, Yu HK, Crum CP, McKeon F, Xian W |
| Citation(s) |
26040716 |
| Submission date |
Feb 19, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
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| Phone |
8608372486
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| Organization name |
University of Houston
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| Department |
Biology and Biochemistry
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| Street address |
3455 Cullen Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE66749 |
Cloning and Variation of Ground State Intestinal Stem Cells Super [expression & SNP studies] |
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| Relations |
| BioProject |
PRJNA275903 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66115_RAW.tar |
39.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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