|
| Status |
Public on Apr 12, 2015 |
| Title |
Regulation of T cell cytokine expression by Tet2-mediated DNA demethylation [MeDIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
T cell function is regulated by epigenetic mechanisms. 5-methylcytosine (5mC) conversion to 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation (Tet) proteins was identified to mediate DNA demethylation. Here, we characterize the genome-wide distribution of 5hmC in T cells using DNA immunoprecipitation coupled with high-throughput DNA sequencing. 5hmC marks signature genes associated with effector cell differentiation in the putative regulatory elements. Moreover, Tet2 protein is recruited to 5hmC-containing regions, dependent on lineage-specific transcription factors. Deletion of the Tet2 gene in T cells decreased their cytokine expression, associated with reduced p300 recruitment. In vivo, Tet2 plays a critical role in the expression of cytokine genes. Collectively, our findings for the first time demonstrate a key role of Tet-mediated active DNA demethylation in T cells.
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| |
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| Overall design |
Examination of 5mC and 5hmC patterns in helper T cell subsets including naïve, Th1, Th2, Th17, and iTreg cells.
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| |
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| Contributor(s) |
Ichiyama K, Wei L, Dong C |
| Citation(s) |
25862091 |
| Submission date |
Feb 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Raymond Doty |
| E-mail(s) |
rtdoty@u.washington.edu
|
| Organization name |
University of Washington
|
| Department |
Division of Hematology
|
| Street address |
Box 357710
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE66945 |
Regulation of T cell cytokine expression by Tet2-mediated DNA demethylation |
|
| Relations |
| BioProject |
PRJNA276287 |
| SRA |
SRP055480 |
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