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| Status |
Public on Feb 26, 2015 |
| Title |
CAGE-Seq of gonads and carcasses from D. melanogaster and D. pseudoobscura |
| Project |
modENCODE
|
| Organisms |
Drosophila melanogaster; Drosophila pseudoobscura |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed CAGE-Seq on dissected ovaries and testes as well as female and male carcasses of two species of Drosophila (D. melanogaster and D. pseudoobscura). These data are used to map transcription start sites.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| Overall design |
For both species, D. melanogaster and D. pseudoobscura, CAGE-Seq was performed on dissected gonads (with reproductive tracts) and remaining carcasses from wild-type adults, using Illumina GAIIx (SRA Study SRP001602, Bioproject PRJNA36315). There are eight samples. Only Drosophila melanogaster testis samples were performed in biological duplicate. See Chen ZX et al. Genome Res 2014, 24(7):1209-23.
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| Contributor(s) |
Chen Z, Oliver B |
| Citation(s) |
24985915 |
| BioProject |
PRJNA200701 |
| Submission date |
Feb 25, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Oliver |
| E-mail(s) |
briano@nih.gov
|
| Phone |
301-204-9463
|
| Organization name |
NIDDK, NIH
|
| Department |
LBG
|
| Lab |
Developmental Genomics
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL17883 |
Illumina Genome Analyzer IIx (Drosophila pseudoobscura) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE44612 |
Comparative Validation of the D. melanogaster Encyclopedia of DNA Elements Transcript Models |
|
| Relations |
| SRA |
SRP001602 |
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