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| Status |
Public on Aug 12, 2018 |
| Title |
DNA methylation profiling of neuron and glia for the dissection of cell type, age and Alzheimer’s disease-specific changes in the human brain |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Alzheimer’s disease (AD) is a progressive brain disorder caused by altered neuronal and glial cell functions. Illumina 450K profiling of cells (nuclei) sorted from post- mortem human brains allowed us assigning cell type- specific epigenetic changes to aging and AD progression. Among a few thousand cell type-specific differentially methylated CpGs (DMCGs) changing with age we identify prominent clusters in the AD genes, CLU, SYNJ2, ANK1 and MCF2L. A distinct set of age independent AD-specific DMCGs shows strong cell specific changes in the genes TOLLIP, BACE2, PKAR1B, PDE9A, ROBO2, FERMT2, UGT8, SPON1, INPP5D, LNFG and SRPK2. Some AD-specific DMCGs even reveal incremental changes with advancing BRAAK stages. In summary we observe a striking presence of DMCGs in major LOAD genes establishing novel connections between genetic and epigenetic contributions to AD. In addition the cell-specific association allows to functionally discriminate epigenetic effects of aging from those related to AD in the human brain.
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| Overall design |
Bulk and cell sorted post-mortem frontal cortex samples
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| Contributor(s) |
Gasparoni G, Bultmann S, Lutsik P, Kraus T, Vlcek J, Dietinger V, Steinmaurer M, Riemenschneider M, Kretzschmar HA, Giehse A, Leonhardt H, Walter J |
| Citation(s) |
30045751 |
| Submission date |
Feb 26, 2015 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Pavlo Lutsik |
| E-mail(s) |
pavlo.lutsik@gmail.com
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| Phone |
04906813022881
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| Organization name |
Saarland University
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| Lab |
EpiGenetics
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| Street address |
Campus A2.4, 0.04
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| City |
Saarbruecken |
| ZIP/Postal code |
66123 |
| Country |
Germany |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (190)
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| Relations |
| BioProject |
PRJNA276585 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66351_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of IDAT) |
| Processed data included within Sample table |
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