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| Status |
Public on Mar 02, 2016 |
| Title |
ChIP-seq validation of recombinant antibodies to histone post-translational modifications |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Recombinant antibodies to histone post-translational modifications (PTMs), with their essentially infinite renewability, could fundamentally eliminate a major source of low reproducibility in epigenetics research. Here, we report new recombinant antibodies to trimethylated Lys4 and Lys9, respectively, on histone H3. Quantitative characterization demonstrated their exquisite specificity and high affinity, and they performed well in common epigenetics applications, including ChIP. These results demonstrate the feasibility of generating recombinant antibodies to a range of histone marks, which will accelerate epigenetics research.
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| |
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| Overall design |
We characterized recombinant antibodies with native ChIP using HEK293 cells followed by deep sequencing.
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| |
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| Contributor(s) |
Hattori T, Grzybowski AT, Ruthenburg AJ, Koide S |
| Citation(s) |
26862167 |
| Submission date |
Mar 04, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Takamitsu Hattori |
| Organization name |
The University of Chicago
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Koide lab
|
| Street address |
900 E. 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA277206 |
| SRA |
SRP055826 |
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