|
| Status |
Public on Mar 11, 2015 |
| Title |
SF3B1 Degron knockdown RNA-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Knockdown of mutant and/or wild-type SF3B1 in MEL202 cell line by Degron knock-in, followed by RNA-seq, to identify splicing events governed by mutant SF3B1.
|
| |
|
| Overall design |
Control: parental MEL202 cell line. Experiments: mutant-SF3B1 knockdown; wildtype-SF3B1 knockdown; mutant SF3B1 knockout. Treatments: each of these four conditions plus and minus shld.
|
| |
|
| Contributor(s) |
Derti A, Zhou Q, Stegmeier F, Ruddy D |
| Citation(s) |
25788694 |
| Submission date |
Mar 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Adnan Derti |
| E-mail(s) |
adnan.derti@gmail.com
|
| Organization name |
personal
|
| Department |
Bioinformatics
|
| Street address |
100 Alden Street
|
| City |
Dedham |
| State/province |
MA |
| ZIP/Postal code |
02026 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (8)
|
| GSM1630516 |
Mel202 parental cells repeat #1 plus Shld |
| GSM1630517 |
Mel202 parental cells repeat #1 minus Shld |
| GSM1630518 |
Mel202 mut-SF3B1 KO plus Shld (2 days) |
| GSM1630519 |
Mel202 mut-SF3B1 KO minus Shld (2 days) |
| GSM1630520 |
Mel202 DD-mut-SF3B1 plus Shld (2 days) |
| GSM1630521 |
Mel202 DD-mut-SF3B1 minus Shld (2 days) |
| GSM1630522 |
Mel202 DD-wt-SF3B1 plus Shld (2 days) |
| GSM1630523 |
Mel202 DD-wt-SF3B1 minus Shld (2 days) |
|
| Relations |
| BioProject |
PRJNA277802 |
| SRA |
SRP056033 |
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