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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 07, 2008 |
| Title |
Expression data during stem cell differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Stem cell development requires selection of specific genetic programs to direct cellular fate. Using microarray technology, we profile expression trends at selected timepoints during stem cell differentiation to characterize these changes.
Keyword(s): timecourse
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| Overall design |
Total RNA was isolated using the Micro-to-Midi isolation kit (Invitrogen), and subjected to comparative gene expression profiling by labelled cRNA hybridization to the mouse genome 430 2.0 microarray (Affymetrix). Data acquired using the GeneChip Scanner 3000 was analyzed with the Genespring GX 7.3 microarray data software bioinformatics suite (Agilent Technologies) restricting the derived gene list to identify differentially expressed genes defined by a >1.5-fold difference and P<0.05. Data population sets were normalized to the undifferentiated phenotype, and quality filtered to eliminate background noise prior to hierarchical clustering.
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| Contributor(s) |
Behfar A, Perez-Terzic C, Faustino RS, Arrell DK, Hodgson D, Yamada S, Puceat M, Niederlander N, Alekseev A, Zingman L, Terzic A |
| Citation(s) |
18184438 |
| Submission date |
Jan 09, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Atta Behfar |
| E-mail(s) |
behfar.atta@mayo.edu
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| Organization name |
Mayo Clinic
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| Street address |
200 First Street SW
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| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA99069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6689_RAW.tar |
81.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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