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| Status |
Public on Mar 23, 2016 |
| Title |
KDM5 Lysine Demethylases Are Involved in Maintenance of 3'UTR Length [ChIP-Seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We discovered that the Saccharomyces cerevisiae lysine demethylase, Jhd2 (also known as KDM5), recruits 3'UTR processing machinery and promotes alteration of 3'UTR length in a demethylase-dependent manner. Interaction of Jhd2 with both chromatin and RNA suggests that Jhd2 affects selection of polyadenylation sites through a transcription-coupled mechanism.
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| |
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| Overall design |
Wild-type yeast or yeast with JHD2 deleted were grown to mid-log phase. ChIP analysis performed for H3K4me3, Pol2, IgG.
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| |
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| Contributor(s) |
Blair LP, Liu Z, Yan Q |
| Citation(s) |
28138513 |
| Submission date |
Mar 24, 2015 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Zongzhi Liu |
| E-mail(s) |
zongzhi.liu@yale.edu
|
| Phone |
2039032599
|
| Organization name |
yale university
|
| Street address |
310 Cedar Street
|
| City |
new haven |
| State/province |
CONNECTICUT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE67212 |
KDM5 Lysine Demethylases Are Involved in Maintenance of 3'UTR Length |
|
| Relations |
| BioProject |
PRJNA279268 |
| SRA |
SRP056484 |
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