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| Status |
Public on Mar 31, 2015 |
| Title |
MicroRNA (miRNA) expression profile in Huh7 cells treated with siRNA targeting nuclear factor 90 (siNF90) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Our previous study has showed that complex of NF90 and nuclear factor 45 (NF45) (NF90-NF45) inhibits miRNA biogenesis through negative regulation of primary-miRNA processing step. On the other hand, miRNAs, the biogenesis of which is regulated by NF90-NF45 in hepatocellular carcinoma, are not clear. Thus, to identify the miRNAs, we performed a miRNA array using RNAs extracted from control Huh7 cells and the cells depleted of NF90.
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| Overall design |
Comparison of miRNA expression profile in one non-targeting control siRNA (siNTC)- or two independent siNF90-treated Huh7 cells.
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| Citation(s) |
27519414 |
| Submission date |
Mar 30, 2015 |
| Last update date |
Sep 21, 2016 |
| Contact name |
Shuji Sakamoto |
| E-mail(s) |
sshuji@kochi-u.ac.jp
|
| Organization name |
Kochi University
|
| Department |
Science Research Center
|
| Lab |
Laboratory of Molecular Biology
|
| Street address |
Oko-cho, Kohasu
|
| City |
Nankoku |
| State/province |
Kochi |
| ZIP/Postal code |
783-8505 |
| Country |
Japan |
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| Platforms (1) |
| GPL8227 |
Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA279846 |
