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Status |
Public on Apr 29, 2015 |
Title |
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells [seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Using an unbiased chemical biology approach, we discover harmine as a novel regulator of Treg/Th17 differentiation. Harmine enhances Treg differentiation (working in conjunction with low levels of exogenous TGFb) and inhibits Th17 differentiation. Analysis of global gene expression of Tregs generated using low TGFb + harmine reveals significant similarity to Tregs generated using high TGFb only and suggests relevance of harmine-engaged mechanisms to IBD.
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Overall design |
Naïve murine CD4+CD62L+ T cells were stimulated with anti-CD3/28, transduced with control or Dyrk1a shRNA, and treated with harmine or control.
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Contributor(s) |
Khor B, Goel G, Schirmer M, Xavier RJ |
Citation(s) |
25998054 |
Submission date |
Apr 16, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Bernard Khor |
E-mail(s) |
bkhor@partners.org
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Organization name |
Massachusetts General Hospital
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Department |
Pathology
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Lab |
Xavier
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Street address |
185 Cambridge St MCPZN 7106
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
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Platforms (1) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE67961 |
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells |
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Relations |
BioProject |
PRJNA281380 |
SRA |
SRP057264 |