|
| Status |
Public on Mar 08, 2017 |
| Title |
Gene expression profile for male SD Rats with and without traumatic brain injury (TBI) by RNA-Seq (CA1 and WBC) |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the effects of TBI on affecting the gene expressions in the hippocampus of male SD rats by RNA-Seq
|
| |
|
| Overall design |
Male Sprague–Dawley (SD) rats weighing between 200 and 240 g were housed in cages and maintained in environmentally-controlled rooms (22–24C) with a 12-h light/dark cycle. After acclimatization for 1 week on standard rat chow, the rats were subjected to TBI by fluid percussion injury (FPI) or sham surgery. At 1 week post-surgery the rats were tested for learning abilities, and then were sacrificed by decapitation. The fresh tissues including the white blood cells (WBC) isolated from blood, and the Region I of hippocampus proper (CA1) dissected were flash frozen, and stored at -70°C for later transcriptome and DNA methylome sequencing experiments. All experiments were performed in accordance with the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the University of California at Los Angeles Chancellor’s Animal Research Committee.
|
| |
|
| Contributor(s) |
Meng Q, Yang X, Gomez-Pinilla F |
| Citation(s) |
28174132 |
| Submission date |
Apr 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xia Yang |
| E-mail(s) |
xyang123@ucla.edu
|
| Organization name |
UCLA
|
| Department |
IBP
|
| Street address |
2000 Terasaki Life Sciences Bldg, 610 Charles E. Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA282032 |
| SRA |
SRP057603 |
Less...
More...