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| Status |
Public on Apr 25, 2015 |
| Title |
Differentially expressed miRNAs in H2AX knockdown cells undergoing apoptosis |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
H2AX has been characterized as a novel tumor suppressor protein. Difficiency of H2AX will result in apoptotic inhibition of cancer cells. However, how H2AX epigenetically regulates apoptosis of cancer cells is still unclear
To reveal the miRNA expression regulated by H2AX and involved in apoptosis, the microarray profiling analysis was employed to identify differentially expressed miRNAs in H2AX knockdown lung cancer cells and control ones after apoptotic induction. H2AX was knockdown by miRNA interfering system in human lung cancer originated cell A549 and the stable cell line named P1, while the control stable cell line named C. Genes with greater than 1.5-fold change and P-value ＜0.05 were identified as differentially expressed genes between C and P1 cells both with apoptosis induction.
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| Overall design |
The two groups including control (C) and H2AX knockdown lung cancer cells (P1) were harvested 48 h after VP-16 treatment. Three independent experiments were performed for each group.
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| Contributor(s) |
Lu C |
| Citation missing |
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| Submission date |
Apr 24, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Chengrong Lu |
| E-mail(s) |
luchengrong@263.net
|
| Organization name |
General Hospital of Air Force
|
| Department |
Aviation Medicine Research lab
|
| Street address |
Fucheng road 30
|
| City |
Beijing |
| ZIP/Postal code |
100142 |
| Country |
China |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA282083 |
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