|
| Status |
Public on May 19, 2015 |
| Title |
Role of cervicovaginal microbiota in genital inflammation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed gene expression analysis using the SCRB-Seq method from cervical and peripheral blood antigen presenting cells that were collected from women with different cervicovaginal bacterial community types.
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| |
|
| Overall design |
Cervical and peripheral blood antigen presenting cells were FACS sorted using the gating strategy: FSC vs. SSC -> Singlets -> Live CD45+ -> HLA-DR+ -> CD11c+ or CD14+. The genital bacterial microbiomes were characterized in the same women and categorized into Lactobacillus dominance, Gardnerella dominance, or Prevotella dominance.
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| |
|
| Contributor(s) |
Anahtar MN, Soumillon M |
| Citation(s) |
25992865 |
| Submission date |
Apr 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Melis Anahtar |
| E-mail(s) |
melis_anahtar@hms.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Ragon Institute of MGH, MIT, Harvard
|
| Lab |
Kwon Lab
|
| Street address |
400 Technology Sq
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA282757 |
| SRA |
SRP057852 |
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