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| Status |
Public on Sep 23, 2015 |
| Title |
High salt primes a specific activation state of macrophages, M(Na) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Investigate effects of high salt on human macrophage activation
Methods: Human monocytes-derived macrophages were treated by additional 51mM NaCl for 24 hours (NaCl groups) or not (Control groups). mRNA profiles were generated by RNA-Seq, in triplicate, using Ion proton（Life tech). qRT–PCR validation was performed using SYBR Green assays.
Results: High salt significantly promotes pro-inflammatory gene expressions,while suppresses the expressions of anti-inflammatory genes and pro-endocytic genes in human macrophages.
Conclusions: Our results identify a novel macrophage activation state, M(Na), and high salt as a potential environmental risk factor for lung inflammation through the induction of M(Na)
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| Overall design |
Human monocytes-derived macrophages were treated by additional 51mM NaCl for 24 hours (NaCl groups) or not (Control groups). mRNA profiles were generated by RNA-Seq, in triplicate, using Ion proton（Life tech). qRT–PCR validation was performed using SYBR Green assays.
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| Contributor(s) |
Zhang W, Duan S |
| Citation(s) |
26206316 |
| Submission date |
May 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
WUCHANG ZHANG |
| E-mail(s) |
zhangwuchang104@hotmail.com
|
| Phone |
4243853408
|
| Organization name |
UCLA
|
| Department |
School of Dentistry
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| Street address |
CHS 56-200, 650 Charles E. Young Dr. So
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA282883 |
| SRA |
SRP057945 |
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