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| Status |
Public on Jun 15, 2007 |
| Title |
A dominant negative form of cJun affects genes that have opposing effects on lipid homeostasis in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
cJun is a transcription factor activated by phosphorylation by SAPK/JNK MAP kinase pathway that has been linked to atherosclerosis. Adenovirus mediated gene transfer of a dominant negative form of cJun in C57BL/6 mice increased greatly the apolipoprotein E (ApoE) mRNA and plasma apoE levels and induced dyslipidmia, characterized by increased plasma cholesterol, triglyceride and VLDL levels and accumulation of discoidal HDL particles. Unexpectedly, infection of ApoE-/- mice with adenovirus expressing dn-cJun reduced by 50% plasma cholesterol, suggesting that the dn-cJun affected other genes that control plamsa cholesterol. To determine the molecular pathways implicated in this process we performed whole genome expression profiling using total RNA from the liver of infected ApoE-/- mice.
Keywords: disease
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| Overall design |
We extracted total RNA from 5 ApoE-/- mice infected with an adenovirus expressing dominant negative cJun and 5 ApoE-/- mice infected with a control adenovirus expressing GFP. Each RNA sample was hybridized to a different Affymetrix 430 2.0 array.
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| Contributor(s) |
Drosatos K, Sanoudou D, Kypreos K, Kardassis D, Zannis V |
| Citation(s) |
17456467 |
| Submission date |
Jan 24, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Despina Sanoudou |
| E-mail(s) |
dsanoudo@enders.tch.harvard.edu
|
| Phone |
+30-210-6597453
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| Fax |
+30-210-6597545
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| Organization name |
Foundation for Biomedical Research, Academy of Athens
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| Department |
Molecular Biology
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| Lab |
Sanoudou Lab
|
| Street address |
Soranou Efesiou 4
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| City |
Athens |
| ZIP/Postal code |
115 27 |
| Country |
Greece |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA99091 |
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