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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 08, 2016 |
| Title |
Cell density, Her2 and progesterone signaling regulate dissemination of breast cancer cells [miRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Disseminated breast cancer cells display genotypes disparate from the predominant clone of the primary tumor before manifestation of metastasis, suggesting that cancer cell dissemination occurs preferentially early; however, the underlying molecular mechanisms are unknown. Investigating metastasis in a Her2-driven mouse model, we found that the progesterone-induced paracrine cytokines Wnt4 and Rankl induced migration and early dissemination shortly after Her2 activation. Once tumorigenic growth was established, progesterone receptor (PgR) expression was lost and Wnt4/Rankl induced proliferation. The altered response from migration to proliferation was determined by cell density involving miRNA-regulated PgR expression and was reversible. Cells from early, low-density lesions displayed more functional stemness traits than cells from dense, advanced tumors, migrated more and founded significantly more metastases. The data suggest that many metastases are derived from early-disseminated cancer cells, implying that our concepts for systemic therapy need to be revised.
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| Overall design |
We analysed miRNA expression in the Her2 expressing cell lines as well as the secreting miRNA containing particles to find out PgR regulating miRNAs
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| Contributor(s) |
Hosseini H, Hoffmann M, Meister G, Klein CA |
| Citation(s) |
27974799 |
| Submission date |
May 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Christoph Andreas Klein |
| Organization name |
University of Regensburg
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| Department |
Experimental Medicine and Therapy Research
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| Street address |
Franz-Josef-Strauß-Allee 11
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| City |
Regensburg |
| State/province |
Bavaria |
| ZIP/Postal code |
93053 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (15)
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| GSM1678990 |
MM3MG, replicate #1 |
| GSM1678991 |
MM3MG, replicate #2 |
| GSM1678992 |
MM3MG, replicate #3 |
| GSM1678993 |
MM3MG-Her2, replicate #1 |
| GSM1678994 |
MM3MG-Her2, replicate #2 |
| GSM1678995 |
MM3MG-Her2, replicate #3 |
| GSM1678996 |
Supernatant of TUBO cells, replicate #1 |
| GSM1678997 |
Supernatant of TUBO cells, replicate #2 |
| GSM1678998 |
Supernatant of TUBO cells, replicate #3 |
| GSM1678999 |
TUBO, replicate #1 |
| GSM1679000 |
TUBO, replicate #2 |
| GSM1679001 |
TUBO, replicate #3 |
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| This SubSeries is part of SuperSeries: |
| GSE68683 |
Cell density, Her2 and progesterone signaling regulate dissemination of breast cancer cells |
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| Relations |
| BioProject |
PRJNA283389 |
| SRA |
SRP058132 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68682_DeepSeq-Results_Summary_Hosseini_Klein.xlsx |
271.2 Kb |
(ftp)(http) |
XLSX |
| GSE68682_RAW.tar |
4.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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