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Status |
Public on Aug 28, 2017 |
Title |
RNA-seq gene expression profiling in resting murine B cells from WT or KMT2D knockout mice, before and after stimulation with LPS, IL4, and anti-CD180 |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Resting B cells were isolated from WT or KMTD KO mice by immunomagentic depletion of with anti-CD43 beads. Gene expression use determined by RNAseq in resting B cells or B cells stimulated with LPS, IL4, and anti-mouse CD180 for 3 days.
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Overall design |
RNAseq was used to profile unstimulated resting B cells (n=3) and B cells stimulated for 3 days (n=3).
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Contributor(s) |
Canela A, Boss IW, Pan H, Melnick A, Nussenzweig A |
Citation(s) |
26366710 |
Submission date |
May 12, 2015 |
Last update date |
Dec 16, 2019 |
Contact name |
Yanwen Jiang |
E-mail(s) |
yaj2001@med.cornell.edu
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Organization name |
Weill Cornell Medical College
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Street address |
413 E. 69th St. BB1462
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (8)
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Relations |
SRA |
SRP058264 |
BioProject |
PRJNA283823 |