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| Status |
Public on Jun 25, 2015 |
| Title |
ATF4 Licenses C/EBPb Activity in Human Mesenchymal Stem Cells Primed for Adipogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report ChIP-seq for C/EBPb and ATF4 in human mesenchymal stem cells and in a cell-free system using naked genomic DNA. ChIP-Seq for GR, RNA Polymerase II, and H3K27 acetylation in hMSCs cultured under different adipogenic conditions are also presented.
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| Overall design |
hMSCs cultured at high or low cell seeding densities in the presence or absence of adipogenic induction cocktail
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| Contributor(s) |
Cohen D, Steger D |
| Citation(s) |
26111340 |
| Submission date |
May 14, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
David J Steger |
| E-mail(s) |
stegerdj@pennmedicine.upenn.edu
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| Organization name |
University of Pennsylvania
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| Department |
Institute of Diabetes, Obesity, and Metabolism
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| Lab |
Steger
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| Street address |
12-193 SCTR 3400 Civic Center Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (3) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (22)
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| GSM1684633 |
ChIP-seq, RNAPII_HighDensity_DMI |
| GSM1684634 |
ChIP-seq, CEBPb_HighDensity_noDMI |
| GSM1684635 |
ChIP-seq, CEBPb_LowDensity_DMI |
| GSM1684636 |
ChIP-seq, CEBPb_HighDensity_DMI |
| GSM1684637 |
ChIP-seq, GR_HighDensity_DMI |
| GSM1684638 |
ChIP-seq, ATF4_HighDensity_noDMI |
| GSM1684639 |
ChIP-seq, H3K27Ac_HighDensity_noDMI |
| GSM1684640 |
ChIP-seq, InVitroCistromics_CEBPb_0.1uL |
| GSM1684641 |
ChIP-seq, InVitroCistromics_CEBPb_0.25uL |
| GSM1684642 |
ChIP-seq, InVitroCistromics_CEBPb_1.0uL |
| GSM1684643 |
ChIP-seq, InVitroCistromics_CEBPb_10uL |
| GSM1684644 |
ChIP-seq, InVitroCistromics_ATF4_5uL |
| GSM1684645 |
ChIP-seq, InVitroCistromics_CEBPb_0.1uL_plusATF4_0.002uL |
| GSM1684646 |
ChIP-seq, InVitroCistromics_CEBPb_0.1uL_plusATF4_0.02uL |
| GSM1684647 |
ChIP-seq, InVitroCistromics_CEBPb_0.1uL_plusATF4_0.2uL |
| GSM1684648 |
ChIP-seq, InVitroCistromics_ATF4_0.02uL_plusCEBPb_0.1uL |
| GSM1684649 |
ChIP-seq, InVitroCistromics_ATF4_0.2uL_plusCEBPb_0.1uL |
| GSM1684650 |
input, InVitroCistromics |
| GSM1684651 |
input, hMSC extract for ChIP-Seq |
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| Relations |
| BioProject |
PRJNA283934 |
| SRA |
SRP058336 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68864_RAW.tar |
445.0 Mb |
(http)(custom) |
TAR (of BED, BEDGRAPH) |
| GSE68864_RNAPII.peaks.bed.gz |
1.1 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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